Line FRAP with the Confocal Laser Scanning Microscope for Diffusion Measurements in Small Regions of 3-D Samples

We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more su...

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Bibliographic Details
Main Authors: Braeckmans, Kevin, Remaut, Katrien, Vandenbroucke, Roosmarijn E., Lucas, Bart, De Smedt, Stefaan C., Demeester, Joseph
Format: Artigo
Language:Inglês
Published: Biophysical Society 2007
Subjects:
Spectroscopy, Imaging, Other Techniques
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1861797/
https://ncbi.nlm.nih.gov/pubmed/17208970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.099838
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