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Comparison of a Reporter Assay and Immunomagnetic Separation Real-Time Reverse Transcription-PCR for the Detection of Enteroviruses in Seeded Environmental Water Samples

Two newly developed protocols for infective virus detection were compared to the plaque assay. An immunomagnetic separation procedure coupled with real-time reverse transcription-PCR of viral nucleic acids was developed to identify intact enteroviral particles, and a reporter cell system responding...

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Sonraí Bibleagrafaíochta
Main Authors: Hwang, Yu-Chen, Leong, Oymon M., Chen, Wilfred, Yates, Marylynn V.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2007
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1855679/
https://ncbi.nlm.nih.gov/pubmed/17277214
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01758-06
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