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Immunomagnetic Separation Combined with Real-Time Reverse Transcriptase PCR Assays for Detection of Norovirus in Contaminated Food

We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. Th...

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Bibliografski detalji
Glavni autori: Park, YoungBin, Cho, You-Hee, Jee, YoungMee, Ko, GwangPyo
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2008
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446532/
https://ncbi.nlm.nih.gov/pubmed/18441102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00013-08
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