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A Derepression System Based on the Bacillus subtilis Sporulation Pathway Offers Dynamic Control of Heterologous Gene Expression

By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-β...

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Detalhes bibliográficos
Main Authors: Nijland, Reindert, Veening, Jan-Willem, Kuipers, Oscar P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1855636/
https://ncbi.nlm.nih.gov/pubmed/17293533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02266-06
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