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Detection of Low-Copy-Number Genomic DNA Sequences in Individual Bacterial Cells by Using Peptide Nucleic Acid-Assisted Rolling-Circle Amplification and Fluorescence In Situ Hybridization

An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybrid...

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Détails bibliographiques
Auteurs principaux: Smolina, Irina, Lee, Charles, Frank-Kamenetskii, Maxim
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2007
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1855634/
https://ncbi.nlm.nih.gov/pubmed/17293504
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02038-06
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