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Direct detection of mRNA expression in microbial cells by fluorescence in situ hybridization using RNase H-assisted rolling circle amplification

Meta-analyses using next generation sequencing is a powerful strategy for studying microbiota; however, it cannot clarify the role of individual microbes within microbiota. To know which cell expresses what gene is important for elucidation of the individual cell’s function in microbiota. In this re...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Takahashi, Hirokazu, Horio, Kyohei, Kato, Setsu, Kobori, Toshiro, Watanabe, Kenshi, Aki, Tsunehiro, Nakashimada, Yutaka, Okamura, Yoshiko
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7295810/
https://ncbi.nlm.nih.gov/pubmed/32541674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-65864-7
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