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Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron (“Targetron”) Expressed from a Broad-Host-Range Vector

Mobile group II introns (“targetrons”) can be programmed for insertion into virtually any desired DNA target with high frequency and specificity. Here, we show that targetrons expressed via an m-toluic acid-inducible promoter from a broad-host-range vector containing an RK2 minireplicon can be used...

詳細記述

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書誌詳細
主要な著者: Yao, Jun, Lambowitz, Alan M.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2007
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1855620/
https://ncbi.nlm.nih.gov/pubmed/17322321
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02829-06
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