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Real-Time Quantification of RNA Polymerase Activity using a “Broken Beacon”

A novel assay was developed for real-time monitoring of RNA synthesis using a hybridization-based method. In this work, a “broken beacon” in which the fluor and quencher were located on separate but complementary oligonucleotides was used to quantify the amount of RNA production by T7 polymerase. Th...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Blair, Rebecca H., Rosenblum, Eric S., Dawson, Erica D., Kuchta, Robert D., Kuck, Laura R., Rowlen, Kathy L.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2006
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC1851671/
https://ncbi.nlm.nih.gov/pubmed/17222795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.12.012
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