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Real-Time Quantification of RNA Polymerase Activity using a “Broken Beacon”
A novel assay was developed for real-time monitoring of RNA synthesis using a hybridization-based method. In this work, a “broken beacon” in which the fluor and quencher were located on separate but complementary oligonucleotides was used to quantify the amount of RNA production by T7 polymerase. Th...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1851671/ https://ncbi.nlm.nih.gov/pubmed/17222795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.12.012 |
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