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Heterologous gene expression in Lactococcus lactis subsp. lactis: synthesis, secretion, and processing of the Bacillus subtilis neutral protease.

The Bacillus subtilis nprE gene lacking its own promoter sequence was inserted in the lactococcal expression vector pMG36e. Upon introduction of the recombinant plasmid into Lactococcus lactis subsp. lactis strain MG1363, neutral protease activity could be visualized by the appearance of large clear...

Ausführliche Beschreibung

Gespeichert in:
Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: van de Guchte, M, Kodde, J, van der Vossen, J M, Kok, J, Venema, G
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1990
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184806/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2125811/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.9.2606-2611.1990
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