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Heterologous gene expression in Lactococcus lactis subsp. lactis: synthesis, secretion, and processing of the Bacillus subtilis neutral protease.

The Bacillus subtilis nprE gene lacking its own promoter sequence was inserted in the lactococcal expression vector pMG36e. Upon introduction of the recombinant plasmid into Lactococcus lactis subsp. lactis strain MG1363, neutral protease activity could be visualized by the appearance of large clear...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: van de Guchte, M, Kodde, J, van der Vossen, J M, Kok, J, Venema, G
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1990
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184806/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2125811/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.9.2606-2611.1990
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