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Isolation of a single-stranded plasmid from Clostridium acetobutylicum NCIB 6444.

The cryptic plasmid pDM6 was isolated from late exponential-phase cells of Clostridium acetobutylicum NCIB 6444 by either alkaline lysis or electroporation. The application of high voltage during electroporation resulted in higher DNA yield than did the alkaline lysis procedure. However, electropora...

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Bibliografiska uppgifter
I publikationen:Appl Environ Microbiol
Huvudupphov: Kim, A Y, Vertes, A A, Blaschek, H P
Materialtyp: Artigo
Språk:Inglês
Utgiven: American Society for Microbiology (ASM) 1990
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184500/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2383010/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.6.1725-1728.1990
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