Isolation of a single-stranded plasmid from Clostridium acetobutylicum NCIB 6444.

The cryptic plasmid pDM6 was isolated from late exponential-phase cells of Clostridium acetobutylicum NCIB 6444 by either alkaline lysis or electroporation. The application of high voltage during electroporation resulted in higher DNA yield than did the alkaline lysis procedure. However, electropora...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Kim, A Y, Vertes, A A, Blaschek, H P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184500/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2383010/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.6.1725-1728.1990
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