Nonradioactive Screening Method for Isolation of Disease-Specific Probes To Diagnose Plant Diseases Caused by Mycoplasmalike Organisms

DNA fragments of tomato big bud (BB) mycoplasmalike organism (MLO) in diseased periwinkle plants (Catharanthus roseus L.) were cloned to pSP6 plasmid vectors and amplified in Escherichia coli JM83. A nonradioactive method was developed and used to screen for MLO-specific recombinants. Cloned DNA pro...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Lee, Ing-Ming, Davis, Robert E., DeWitt, Natalie D.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1990
Assuntos:	Microorganism-Plant Interactions
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184432/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348195/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.5.1471-1475.1990
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