Nonradioactive Screening Method for Isolation of Disease-Specific Probes To Diagnose Plant Diseases Caused by Mycoplasmalike Organisms

DNA fragments of tomato big bud (BB) mycoplasmalike organism (MLO) in diseased periwinkle plants (Catharanthus roseus L.) were cloned to pSP6 plasmid vectors and amplified in Escherichia coli JM83. A nonradioactive method was developed and used to screen for MLO-specific recombinants. Cloned DNA pro...

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Dades bibliogràfiques

Publicat a:	Appl Environ Microbiol
Autors principals:	Lee, Ing-Ming, Davis, Robert E., DeWitt, Natalie D.
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1990
Matèries:	Microorganism-Plant Interactions
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184432/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348195/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.5.1471-1475.1990
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