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Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus.

SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated.

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Bibliografske podrobnosti
Main Authors: Benbadis, L, Garel, J R, Hartley, D L
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC184036/
https://ncbi.nlm.nih.gov/pubmed/1785940
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