Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus.

SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated. IMAG...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Benbadis, L, Garel, J R, Hartley, D L
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1991
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184036/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1785940/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.12.3677-3678.1991
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