Site-specific mutagenesis method which completely excludes wild-type DNA from the transformants.

A highly efficient site-specific mutagenesis method has been devised to exclude wild-type DNA from incorporation into the transformed cells. Two complementary oligonucleotides, corresponding to a target sequence of a DNA molecule and containing an insertion mutation which created an endonuclease res...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Lee, N, Liu, J, He, C, Testa, D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183891/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1746949/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.10.2888-2890.1991
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