Site-specific mutagenesis method which completely excludes wild-type DNA from the transformants.
A highly efficient site-specific mutagenesis method has been devised to exclude wild-type DNA from incorporation into the transformed cells. Two complementary oligonucleotides, corresponding to a target sequence of a DNA molecule and containing an insertion mutation which created an endonuclease res...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183891/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1746949/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.10.2888-2890.1991 |
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