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Importance of culturing primary lymphocytes at physiological oxygen levels

Although studies with primary lymphocytes are almost always conducted in CO(2) incubators maintained at atmospheric oxygen levels (atmosO(2); 20%), the physiological oxygen levels (physO(2); 5%) that cells encounter in vivo are 2–4 times lower. We show here that culturing primary T cells at atmosO(2...

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Bibliographische Detailangaben
Hauptverfasser: Atkuri, Kondala R., Herzenberg, Leonard A., Niemi, Anna-Kaisa, Cowan, Tina, Herzenberg, Leonore A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2007
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1838638/
https://ncbi.nlm.nih.gov/pubmed/17360561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0611732104
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