Two-Color Far-Field Fluorescence Nanoscopy

We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The s...

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Bibliografiske detaljer
Main Authors: Donnert, Gerald, Keller, Jan, Wurm, Christian A., Rizzoli, Silvio O., Westphal, Volker, Schönle, Andreas, Jahn, Reinhard, Jakobs, Stefan, Eggeling, Christian, Hell, Stefan W.
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2007
Fag:
Biophysical Letters
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1831704/
https://ncbi.nlm.nih.gov/pubmed/17307826
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.104497
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