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Real-Time PCR Diagnostics Failure Caused by Nucleotide Variability within Exon 4 of the Human Cytomegalovirus Major Immediate-Early Gene

Here we report how variability in the human cytomegalovirus genome sequence may seriously affect the outcome of its molecular diagnosis. A real-time quantitative PCR assay targeting the major immediate-early gene failed to detect the viral load in some, but not all, clinical samples from hematooncol...

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Main Authors: Lengerova, Martina, Racil, Zdenek, Volfova, Pavlina, Lochmanova, Jana, Berkovcova, Jitka, Dvorakova, Dana, Vorlicek, Jiri, Mayer, Jiri
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1829136/
https://ncbi.nlm.nih.gov/pubmed/17229861
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01109-06
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