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Development of a Semiquantitative Degenerate Real-Time PCR-Based Assay for Estimation of Numbers of Butyryl-Coenzyme A (CoA) CoA Transferase Genes in Complex Bacterial Samples

We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the hum...

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Bibliografiske detaljer
Main Authors: Louis, Petra, Flint, Harry J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2007
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1828812/
https://ncbi.nlm.nih.gov/pubmed/17259367
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02561-06
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