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Gateway-Based Destination Vectors for Functional Analyses of Bacterial ORFeomes: Application to the Min System in Brucella abortus

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we obser...

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Main Authors: Hallez, Régis, Letesson, Jean-Jacques, Vandenhaute, Jean, De Bolle, Xavier
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1828643/
https://ncbi.nlm.nih.gov/pubmed/17172460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01873-06
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