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Gateway-Based Destination Vectors for Functional Analyses of Bacterial ORFeomes: Application to the Min System in Brucella abortus

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we obser...

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Detalhes bibliográficos
Main Authors: Hallez, Régis, Letesson, Jean-Jacques, Vandenhaute, Jean, De Bolle, Xavier
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1828643/
https://ncbi.nlm.nih.gov/pubmed/17172460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01873-06
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