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Gateway-Based Destination Vectors for Functional Analyses of Bacterial ORFeomes: Application to the Min System in Brucella abortus

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we obser...

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Auteurs principaux: Hallez, Régis, Letesson, Jean-Jacques, Vandenhaute, Jean, De Bolle, Xavier
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2007
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1828643/
https://ncbi.nlm.nih.gov/pubmed/17172460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01873-06
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