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Selecting rRNA binding sites for the ribosomal proteins L4 and L6 from randomly fragmented rRNA: Application of a method called SERF

Two-thirds of the 54 proteins of the Escherichia coli ribosome interact directly with the rRNAs, but the rRNA binding sites of only a very few proteins are known. We present a method (selection of random RNA fragments; SERF) that can identify the minimal binding region for proteins within ribonucleo...

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Main Authors: Stelzl, Ulrich, Spahn, Christian M. T., Nierhaus, Knud H.
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC18278/
https://ncbi.nlm.nih.gov/pubmed/10781065
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