Genetic construction of nisin-producing Lactococcus lactis subsp. cremoris and analysis of a rapid method for conjugation.

Conjugation was used to construct nisin-producing Lactococcus lactis subsp. cremoris strains. Recipients were obtained by electroporation of L. lactis subsp. cremoris strains with the drug resistance plasmid pGK13 or pGB301. A method, direct-plate conjugation, was developed in which donor and recipi...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Broadbent, J R, Kondo, J K
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1991
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182742/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1901708/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.2.517-524.1991
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