Biolistic transformation of a procaryote, Bacillus megaterium.
We present a simple and rapid method for introducing exogenous DNA into a bacterium, Bacillus megaterium, utilizing the recently developed biolistic process. A suspension of B. megaterium was spread onto the surface of nonselective medium. Plasmid pUB110 DNA, which contains a gene that confers kanam...
Uloženo v:
| Vydáno v: | Appl Environ Microbiol |
|---|---|
| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1991
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182736/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1901706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.2.480-485.1991 |
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