Biolistic transformation of a procaryote, Bacillus megaterium.

We present a simple and rapid method for introducing exogenous DNA into a bacterium, Bacillus megaterium, utilizing the recently developed biolistic process. A suspension of B. megaterium was spread onto the surface of nonselective medium. Plasmid pUB110 DNA, which contains a gene that confers kanam...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Shark, K B, Smith, F D, Harpending, P R, Rasmussen, J L, Sanford, J C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182736/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1901706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.2.480-485.1991
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