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Stable Tagging of Rhizobium meliloti with the Firefly Luciferase Gene for Environmental Monitoring
A system for stable tagging of gram-negative bacteria with the firefly luciferase gene, luc, is described. A previously constructed fusion constitutively expressing luc from the λp(R) promoter was used. Stable integration into the bacterial genome was achieved by use of mini-Tn5 delivery vectors. Th...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1993
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC182313/ https://ncbi.nlm.nih.gov/pubmed/16349015 |
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