Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils

This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards...

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Main Authors: Young, Charles C., Burghoff, Robert L., Keim, Lois G., Minak-Bernero, Vera, Lute, James R., Hinton, Stephen M.
Format: Artigo
Jezik:Inglês
Izdano: 1993
Teme:
Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC182198/
https://ncbi.nlm.nih.gov/pubmed/16348974
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