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Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils

This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards...

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Udgivet i:Appl Environ Microbiol
Principais autores: Young, Charles C., Burghoff, Robert L., Keim, Lois G., Minak-Bernero, Vera, Lute, James R., Hinton, Stephen M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1993
Fag:
Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182198/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348974/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.6.1972-1974.1993
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