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Screening poly(dA/dT)(−) cDNAs for gene identification

Many genes expressed in the human genome have not been identified despite intensive efforts. We observed that the presence of long poly(dA/dT) sequences in the 3′ end of cDNA templates contributes significantly to this problem, because the hybrids formed randomly between poly(dA) and poly(dT) sequen...

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Detalhes bibliográficos
Main Authors: Wang, San Ming, Fears, Scott C., Zhang, Lin, Chen, Jian-Jun, Rowley, Janet D.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC18183/
https://ncbi.nlm.nih.gov/pubmed/10760283
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