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Incorporation of 5-fluorouracil into U2 snRNA blocks pseudouridylation and pre-mRNA splicing in vivo

5-fluorouracil (5FU) is an effective anti-cancer drug, yet its mechanism of action remains unclear. Here, we examine the effect of 5FU on pre-mRNA splicing in vivo. Using RT–PCR, we show that the splicing of a number of pre-mRNAs is inhibited in HeLa cells that have been exposed to a low dose of 5FU...

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Sonraí Bibleagrafaíochta
Main Authors: Zhao, Xinliang, Yu, Yi-Tao
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2007
Ábhair:
RNA
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1802618/
https://ncbi.nlm.nih.gov/pubmed/17169984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl1084
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