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Site-specific deoxynucleotide substitutions in yeast U6 snRNA block splicing of pre-mRNA in vitro.

We have identified 2'-hydroxyl groups of the U6 phosphate-ribose backbone which are required for reconstitution of splicing activity in U6-depleted yeast extract. To screen the 2'-hydroxyls of yeast U6 at nucleotides 39-88, spanning the conserved central domain, synthetic U6 RNAs were cons...

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Hlavní autoři: Kim, C H, Ryan, D E, Marciniec, T, Abelson, J
Médium: Artigo
Jazyk:Inglês
Vydáno: 1997
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1169814/
https://ncbi.nlm.nih.gov/pubmed/9155037
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/16.8.2119
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