Effects of Target Length on the Hybridization Efficiency and Specificity of rRNA-Based Oligonucleotide Microarrays

The effect of target size on microarray hybridization efficiencies and specificity was investigated using a set of 166 oligonucleotide probes targeting the 16S rRNA gene of Escherichia coli. The targets included unfragmented native rRNA, fragmented rRNA (∼20 to 100 bp), PCR amplicons (93 to 1,480 bp...

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Bibliographic Details
Main Authors: Liu, Wen-Tso, Guo, Huiling, Wu, Jer-Horng
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2007
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1797121/
https://ncbi.nlm.nih.gov/pubmed/17071797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01468-06
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