Microarray-Based Analysis of Microbial Community RNAs by Whole-Community RNA Amplification

A new approach, termed whole-community RNA amplification (WCRA), was developed to provide sufficient amounts of mRNAs from environmental samples for microarray analysis. This method employs fusion primers (six to nine random nucleotides with an attached T7 promoter) for the first-strand synthesis. T...

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Main Authors: Gao, Haichun, Yang, Zamin K., Gentry, Terry J., Wu, Liyou, Schadt, Christopher W., Zhou, Jizhong
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2007
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Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1796964/
https://ncbi.nlm.nih.gov/pubmed/17098911
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01771-06
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