Generation of medaka gene knockout models by target-selected mutagenesis

We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations...

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Detalhes bibliográficos
Main Authors: Taniguchi, Yoshihito, Takeda, Shunichi, Furutani-Seiki, Makoto, Kamei, Yasuhiro, Todo, Takeshi, Sasado, Takao, Deguchi, Tomonori, Kondoh, Hisato, Mudde, Josine, Yamazoe, Mitsuyoshi, Hidaka, Masayuki, Mitani, Hiroshi, Toyoda, Atsushi, Sakaki, Yoshiyuki, Plasterk, Ronald HA, Cuppen, Edwin
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2006
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1794429/
https://ncbi.nlm.nih.gov/pubmed/17156454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2006-7-12-r116
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