Molecular characterization of glucokinase from Escherichia coli K-12.

glk, the structural gene for glucokinase of Escherichia coli, was cloned and sequenced. Overexpression of glk resulted in the synthesis of a cytoplasmic protein with a molecular weight of 35,000. The enzyme was purified, and its kinetic parameters were determined. Its Km values for glucose and ATP w...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Meyer, D, Schneider-Fresenius, C, Horlacher, R, Peist, R, Boos, W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178829/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023215/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.4.1298-1306.1997
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