Molecular characterization of glucokinase from Escherichia coli K-12.
glk, the structural gene for glucokinase of Escherichia coli, was cloned and sequenced. Overexpression of glk resulted in the synthesis of a cytoplasmic protein with a molecular weight of 35,000. The enzyme was purified, and its kinetic parameters were determined. Its Km values for glucose and ATP w...
Guardat en:
| Publicat a: | J Bacteriol |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1997
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178829/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023215/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.4.1298-1306.1997 |
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