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DNA-binding determinants of sigma 54 as deduced from libraries of mutations.

PCR mutagenesis was used to obtain libraries of mutations in the region between amino acids 300 and 400 in the DNA-binding domain of Escherichia coli sigma 54. Two hundred changes that did not alter function were identified. These were compared with a somewhat smaller number of changes that did alte...

Ausführliche Beschreibung

Gespeichert in:
Veröffentlicht in:J Bacteriol
Hauptverfasser: Guo, Y, Gralla, J D
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1997
Schlagworte:
Research Article
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178821/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023207/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.4.1239-1245.1997
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