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DNA-binding determinants of sigma 54 as deduced from libraries of mutations.

PCR mutagenesis was used to obtain libraries of mutations in the region between amino acids 300 and 400 in the DNA-binding domain of Escherichia coli sigma 54. Two hundred changes that did not alter function were identified. These were compared with a somewhat smaller number of changes that did alte...

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Udgivet i:J Bacteriol
Principais autores: Guo, Y, Gralla, J D
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178821/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023207/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.4.1239-1245.1997
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