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Distance Measurements by Fluorescence Energy Homotransfer: Evaluation in T4 Lysozyme and Correlation with Dipolar Coupling between Spin Labels
We demonstrate the feasibility and practical limitations of using steady-state anisotropy to determine distances from fluorescence homotransfer in the context of a protein of known crystal structure. Eight double mutants of T4 lysozyme spanning the distance range between 20 Å and 50 Å were labeled w...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Biophysical Society
2007
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1783887/ https://ncbi.nlm.nih.gov/pubmed/17142264 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.098913 |
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