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Multiple-peptidase mutants of Lactococcus lactis are severely impaired in their ability to grow in milk.

To examine the contribution of peptidases to the growth of lactococcus lactis in milk, 16 single- and multiple-deletion mutants were constructed. In successive rounds of chromosomal gene replacement mutagenesis, up to all five of the following peptidase genes were inactivated (fivefold mutant): pepX...

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Udgivet i:J Bacteriol
Principais autores: Mierau, I, Kunji, E R, Leenhouts, K J, Hellendoorn, M A, Haandrikman, A J, Poolman, B, Konings, W N, Venema, G, Kok, J
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178013/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8631666/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.10.2794-2803.1996
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