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Temperature Effects on DNA Chip Experiments from Surface Plasmon Resonance Imaging: Isotherms and Melting Curves

We present an analysis of hybridization experiments on a DNA chip studied by surface plasmon resonance imaging. The reaction constants at various temperatures and for different probe lengths are obtained from Langmuir isotherms and hybridization kinetics. The melting curves from temperature scans ar...

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Detalhes bibliográficos
Main Authors: Fiche, J. B., Buhot, A., Calemczuk, R., Livache, T.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1779967/
https://ncbi.nlm.nih.gov/pubmed/17085497
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.097790
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