Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase.

We have cloned two open reading frames (orf6 and orf8) from the Escherichia coli K-12 rfb region. The genes were expressed in E. coli under control of the T7lac promoter, producing large quantities of recombinant protein, most of which accumulated in insoluble inclusion bodies. Sufficient soluble pr...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Nassau, P M, Martin, S L, Brown, R E, Weston, A, Monsey, D, McNeil, M R, Duncan, K
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1996
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177764/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8576037/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.4.1047-1052.1996
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