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Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase.

We have cloned two open reading frames (orf6 and orf8) from the Escherichia coli K-12 rfb region. The genes were expressed in E. coli under control of the T7lac promoter, producing large quantities of recombinant protein, most of which accumulated in insoluble inclusion bodies. Sufficient soluble pr...

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Published in:J Bacteriol
Main Authors: Nassau, P M, Martin, S L, Brown, R E, Weston, A, Monsey, D, McNeil, M R, Duncan, K
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1996
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177764/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8576037/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.4.1047-1052.1996
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