The nucleotide concentration determines the specificity of in vitro transcription activation by the sigma 54-dependent activator FhlA.
An in vitro transcription system has been set up for formate- and FhlA-dependent transcription activation at the -12/-24 promoter of the fdhF gene from Escherichia coli by sigma 54-RNA polymerase. It requires the presence of the upstream activation sequence on supercoiled DNA. Transcription is indep...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1996
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177639/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8550417/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.1.199-203.1996 |
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