The nucleotide concentration determines the specificity of in vitro transcription activation by the sigma 54-dependent activator FhlA.
An in vitro transcription system has been set up for formate- and FhlA-dependent transcription activation at the -12/-24 promoter of the fdhF gene from Escherichia coli by sigma 54-RNA polymerase. It requires the presence of the upstream activation sequence on supercoiled DNA. Transcription is indep...
Gespeichert in:
| Veröffentlicht in: | J Bacteriol |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1996
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177639/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8550417/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.1.199-203.1996 |
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