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Genetics in methanogens: transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene.

We designed a transposon insertion mutagenesis system for Methanococcus species and used it to make mutations in and around a nifH gene in Methanococcus maripaludis. The transposon Mudpur was constructed with a gene for puromycin resistance that is expressed and selectable in Methanococcus species....

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Detalhes bibliográficos
Main Authors: Blank, C E, Kessler, P S, Leigh, J A
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC177397/
https://ncbi.nlm.nih.gov/pubmed/7592322
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